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reovirus strain type 3 dearing t3d  (ATCC)


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    ATCC reovirus strain type 3 dearing t3d
    Reovirus Strain Type 3 Dearing T3d, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 171 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/reovirus+type+3/Reovirus/pmc13078086-43-1-10
    Average 95 stars, based on 171 article reviews
    reovirus strain type 3 dearing t3d - by Bioz Stars, 2026-10
    95/100 stars

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    other:

    Article Title: Integrated Cell Culture-PCR Detection of Enteroviruses and Reoviruses in Water Sources in Gyeonggi-do
    Article Snippet: The integrated cell culture-PCR (ICC-PCR) method has been suggested as an improved method for detection of viruses in water environments.. We tested 57 source waters including finished water samples in Gyeonggi-do for enteric viral contamination using total culturable virus assay (TCVA) using BGMK cells and ICC-PCR.. Nineteen of the 57 source water samples (33.3%) exhibited the cytopathic effect (CPE) on BGMK cells and no finished water did exhibited CPE.

    Virus:

    Article Title: In Vitro Characterization of a Nineteenth-Century Therapy for Smallpox
    Article Snippet: .. Adenovirus Type 5 (Adeno), vesicular stomatitis virus, Indiana strain (VSV), mouse hepatitis virus A59 (MHV), reovirus Type 3, Dearing strain (Reo), encephalomyocarditis virus (EMCV) were obtained from American Type Culture Collection (ATCC). ..

    Article Title: High waste-to-biomass conversion and efficient Salmonella spp. reduction using black soldier fly for waste recycling
    Article Snippet: .. The virus inoculate comprised the following: Reovirus type 3 (ATCC VR-232); strain Abney (human); genus Orthoreovirus; Canine Adenovirus I, strain vacc-98, genus Mastadenovirus; and Porcine enterovirus I, genus Teschovirus. ..

    Article Title: Inactivation of lipid enveloped viruses by octanoic Acid treatment of immunoglobulin solution.
    Article Snippet: Inactivation of lipid enveloped viruses by treatment with octanoic acid has been investigated for three intravenous immunoglobulin preparations, using Human Immunodeficiency Virus, Bovine Viral Diarrhoea Virus, Sindbis Virus and Pseudorabies Virus as test viruses.. At a concentration of 7·45 g octanoic acid per kg solution complete inactivation of lipid enveloped viruses to below detectable level (>5·36, >4·68, >6·25 and >5·55 log10, respectively) was achieved within the first minutes of treatment.. Octanoic acid treatment as described here, has been demonstrated as an effective and rapid virus inactivation procedure, which shows high robustness at the tested ranges of temperature, pH and protein content of the test material.

    Titration:

    Article Title: Inactivation of lipid enveloped viruses by octanoic Acid treatment of immunoglobulin solution.
    Article Snippet: Inactivation of lipid enveloped viruses by treatment with octanoic acid has been investigated for three intravenous immunoglobulin preparations, using Human Immunodeficiency Virus, Bovine Viral Diarrhoea Virus, Sindbis Virus and Pseudorabies Virus as test viruses.. At a concentration of 7·45 g octanoic acid per kg solution complete inactivation of lipid enveloped viruses to below detectable level (>5·36, >4·68, >6·25 and >5·55 log10, respectively) was achieved within the first minutes of treatment.. Octanoic acid treatment as described here, has been demonstrated as an effective and rapid virus inactivation procedure, which shows high robustness at the tested ranges of temperature, pH and protein content of the test material.



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    reovirus strain type 3 dearing t3d  (ATCC)
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    ATCC reovirus strain type 3 dearing t3d
    Reovirus Strain Type 3 Dearing T3d, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    reovirus type 3  (ATCC)
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    wt type 3 dearing t3d reovirus strain r124  (ATCC)
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    ATCC wt type 3 dearing t3d reovirus strain r124
    ZEB1 KO in human and murine fibroblasts results in increased susceptibility to reovirus infection and reovirus-mediated apoptotic cell death (A, E, and I) Cell viability (%) relative to mock following infection with reovirus at multiple MOIs, as measured by a WST-1 assay. RLT-PSC (A) were infected for 4 days, hPS1 (E) for 6 days, and KPC3-CAF1 (I) for 3 days. Significance was calculated using two-way ANOVA with correction for multiple testing (Šídák’s test), significance is depicted at MOI 10, ∗ p ≤ 0.05, ∗∗ p ≤ 0.01, ∗∗∗∗ p ≤ 0.0001. Data are derived from a representative experiment and plotted as mean ± SD. (B, F, and J) Overlay of phase-contrast and GFP images of CellEvent Caspase 3/7 assay of RLT-PSC (B), hPS1 (F), and KPC3-CAF1 (J) WT and ZEB1 KO cells. Cells were infected with <t>R124</t> MOI 10 for 36, 48, and 24 h, respectively. Scale bars: 200 μM. (C, G, and K) Quantification of CellEvent Caspase 3/7 fluorescent signal over time, during infection with reovirus R124 MOI 10 for 36 (RLT-PSC, C), 48 (hPS1, G), or 24 h (KPC3-CAF1, K). Significance was calculated using two-way ANOVA with correction for multiple testing (Šídák’s test), ∗∗∗∗ p ≤ 0.0001. Data are derived from a representative experiment and plotted as mean ± SD. (D, H, and L) Western blot for reovirus Sigma3 expression (41 kDa) and vinculin (117 kDa) as loading control in WT and ZEB1 KO cells following reovirus infection at different MOIs. RLT-PSC (B) and hPS1 (E) were infected for 2 days and KPC3-CAF1 (H) for 1 day.
    Wt Type 3 Dearing T3d Reovirus Strain R124, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    wild type type 3 dearing t3d reovirus strain r124  (ATCC)
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    ZEB1 KO in human and murine fibroblasts results in increased susceptibility to reovirus infection and reovirus-mediated apoptotic cell death (A, E, and I) Cell viability (%) relative to mock following infection with reovirus at multiple MOIs, as measured by a WST-1 assay. RLT-PSC (A) were infected for 4 days, hPS1 (E) for 6 days, and KPC3-CAF1 (I) for 3 days. Significance was calculated using two-way ANOVA with correction for multiple testing (Šídák’s test), significance is depicted at MOI 10, ∗ p ≤ 0.05, ∗∗ p ≤ 0.01, ∗∗∗∗ p ≤ 0.0001. Data are derived from a representative experiment and plotted as mean ± SD. (B, F, and J) Overlay of phase-contrast and GFP images of CellEvent Caspase 3/7 assay of RLT-PSC (B), hPS1 (F), and KPC3-CAF1 (J) WT and ZEB1 KO cells. Cells were infected with <t>R124</t> MOI 10 for 36, 48, and 24 h, respectively. Scale bars: 200 μM. (C, G, and K) Quantification of CellEvent Caspase 3/7 fluorescent signal over time, during infection with reovirus R124 MOI 10 for 36 (RLT-PSC, C), 48 (hPS1, G), or 24 h (KPC3-CAF1, K). Significance was calculated using two-way ANOVA with correction for multiple testing (Šídák’s test), ∗∗∗∗ p ≤ 0.0001. Data are derived from a representative experiment and plotted as mean ± SD. (D, H, and L) Western blot for reovirus Sigma3 expression (41 kDa) and vinculin (117 kDa) as loading control in WT and ZEB1 KO cells following reovirus infection at different MOIs. RLT-PSC (B) and hPS1 (E) were infected for 2 days and KPC3-CAF1 (H) for 1 day.
    Wild Type Type 3 Dearing T3d Reovirus Strain R124, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    reo type 3 dearing strain  (ATCC)
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    ATCC reo type 3 dearing strain
    ZEB1 KO in human and murine fibroblasts results in increased susceptibility to reovirus infection and reovirus-mediated apoptotic cell death (A, E, and I) Cell viability (%) relative to mock following infection with reovirus at multiple MOIs, as measured by a WST-1 assay. RLT-PSC (A) were infected for 4 days, hPS1 (E) for 6 days, and KPC3-CAF1 (I) for 3 days. Significance was calculated using two-way ANOVA with correction for multiple testing (Šídák’s test), significance is depicted at MOI 10, ∗ p ≤ 0.05, ∗∗ p ≤ 0.01, ∗∗∗∗ p ≤ 0.0001. Data are derived from a representative experiment and plotted as mean ± SD. (B, F, and J) Overlay of phase-contrast and GFP images of CellEvent Caspase 3/7 assay of RLT-PSC (B), hPS1 (F), and KPC3-CAF1 (J) WT and ZEB1 KO cells. Cells were infected with <t>R124</t> MOI 10 for 36, 48, and 24 h, respectively. Scale bars: 200 μM. (C, G, and K) Quantification of CellEvent Caspase 3/7 fluorescent signal over time, during infection with reovirus R124 MOI 10 for 36 (RLT-PSC, C), 48 (hPS1, G), or 24 h (KPC3-CAF1, K). Significance was calculated using two-way ANOVA with correction for multiple testing (Šídák’s test), ∗∗∗∗ p ≤ 0.0001. Data are derived from a representative experiment and plotted as mean ± SD. (D, H, and L) Western blot for reovirus Sigma3 expression (41 kDa) and vinculin (117 kDa) as loading control in WT and ZEB1 KO cells following reovirus infection at different MOIs. RLT-PSC (B) and hPS1 (E) were infected for 2 days and KPC3-CAF1 (H) for 1 day.
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    ZEB1 KO in human and murine fibroblasts results in increased susceptibility to reovirus infection and reovirus-mediated apoptotic cell death (A, E, and I) Cell viability (%) relative to mock following infection with reovirus at multiple MOIs, as measured by a WST-1 assay. RLT-PSC (A) were infected for 4 days, hPS1 (E) for 6 days, and KPC3-CAF1 (I) for 3 days. Significance was calculated using two-way ANOVA with correction for multiple testing (Šídák’s test), significance is depicted at MOI 10, ∗ p ≤ 0.05, ∗∗ p ≤ 0.01, ∗∗∗∗ p ≤ 0.0001. Data are derived from a representative experiment and plotted as mean ± SD. (B, F, and J) Overlay of phase-contrast and GFP images of CellEvent Caspase 3/7 assay of RLT-PSC (B), hPS1 (F), and KPC3-CAF1 (J) WT and ZEB1 KO cells. Cells were infected with <t>R124</t> MOI 10 for 36, 48, and 24 h, respectively. Scale bars: 200 μM. (C, G, and K) Quantification of CellEvent Caspase 3/7 fluorescent signal over time, during infection with reovirus R124 MOI 10 for 36 (RLT-PSC, C), 48 (hPS1, G), or 24 h (KPC3-CAF1, K). Significance was calculated using two-way ANOVA with correction for multiple testing (Šídák’s test), ∗∗∗∗ p ≤ 0.0001. Data are derived from a representative experiment and plotted as mean ± SD. (D, H, and L) Western blot for reovirus Sigma3 expression (41 kDa) and vinculin (117 kDa) as loading control in WT and ZEB1 KO cells following reovirus infection at different MOIs. RLT-PSC (B) and hPS1 (E) were infected for 2 days and KPC3-CAF1 (H) for 1 day.
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    reovirus type 3 sigma1 protein (pσ1)  (ATUM Bio)
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    ATUM Bio reovirus type 3 sigma1 protein (pσ1)
    An external file that holds a picture, illustration, etc. Object name is plantbiotechnology-39-4-22.0926a-figure01.jpg
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    ZEB1 KO in human and murine fibroblasts results in increased susceptibility to reovirus infection and reovirus-mediated apoptotic cell death (A, E, and I) Cell viability (%) relative to mock following infection with reovirus at multiple MOIs, as measured by a WST-1 assay. RLT-PSC (A) were infected for 4 days, hPS1 (E) for 6 days, and KPC3-CAF1 (I) for 3 days. Significance was calculated using two-way ANOVA with correction for multiple testing (Šídák’s test), significance is depicted at MOI 10, ∗ p ≤ 0.05, ∗∗ p ≤ 0.01, ∗∗∗∗ p ≤ 0.0001. Data are derived from a representative experiment and plotted as mean ± SD. (B, F, and J) Overlay of phase-contrast and GFP images of CellEvent Caspase 3/7 assay of RLT-PSC (B), hPS1 (F), and KPC3-CAF1 (J) WT and ZEB1 KO cells. Cells were infected with R124 MOI 10 for 36, 48, and 24 h, respectively. Scale bars: 200 μM. (C, G, and K) Quantification of CellEvent Caspase 3/7 fluorescent signal over time, during infection with reovirus R124 MOI 10 for 36 (RLT-PSC, C), 48 (hPS1, G), or 24 h (KPC3-CAF1, K). Significance was calculated using two-way ANOVA with correction for multiple testing (Šídák’s test), ∗∗∗∗ p ≤ 0.0001. Data are derived from a representative experiment and plotted as mean ± SD. (D, H, and L) Western blot for reovirus Sigma3 expression (41 kDa) and vinculin (117 kDa) as loading control in WT and ZEB1 KO cells following reovirus infection at different MOIs. RLT-PSC (B) and hPS1 (E) were infected for 2 days and KPC3-CAF1 (H) for 1 day.

    Journal: Molecular Therapy Oncology

    Article Title: ZEB1, a novel junctional adhesion molecule A regulator, impacts sensitivity of pancreatic cancer-associated fibroblasts to reovirus

    doi: 10.1016/j.omton.2025.201071

    Figure Lengend Snippet: ZEB1 KO in human and murine fibroblasts results in increased susceptibility to reovirus infection and reovirus-mediated apoptotic cell death (A, E, and I) Cell viability (%) relative to mock following infection with reovirus at multiple MOIs, as measured by a WST-1 assay. RLT-PSC (A) were infected for 4 days, hPS1 (E) for 6 days, and KPC3-CAF1 (I) for 3 days. Significance was calculated using two-way ANOVA with correction for multiple testing (Šídák’s test), significance is depicted at MOI 10, ∗ p ≤ 0.05, ∗∗ p ≤ 0.01, ∗∗∗∗ p ≤ 0.0001. Data are derived from a representative experiment and plotted as mean ± SD. (B, F, and J) Overlay of phase-contrast and GFP images of CellEvent Caspase 3/7 assay of RLT-PSC (B), hPS1 (F), and KPC3-CAF1 (J) WT and ZEB1 KO cells. Cells were infected with R124 MOI 10 for 36, 48, and 24 h, respectively. Scale bars: 200 μM. (C, G, and K) Quantification of CellEvent Caspase 3/7 fluorescent signal over time, during infection with reovirus R124 MOI 10 for 36 (RLT-PSC, C), 48 (hPS1, G), or 24 h (KPC3-CAF1, K). Significance was calculated using two-way ANOVA with correction for multiple testing (Šídák’s test), ∗∗∗∗ p ≤ 0.0001. Data are derived from a representative experiment and plotted as mean ± SD. (D, H, and L) Western blot for reovirus Sigma3 expression (41 kDa) and vinculin (117 kDa) as loading control in WT and ZEB1 KO cells following reovirus infection at different MOIs. RLT-PSC (B) and hPS1 (E) were infected for 2 days and KPC3-CAF1 (H) for 1 day.

    Article Snippet: The WT type 3 Dearing (T3D) reovirus strain R124 was isolated by plaque purification from a heterogenous T3D stock obtained from ATCC (VR-824) and propagated and purified as described previously.

    Techniques: Infection, WST-1 Assay, Derivative Assay, Western Blot, Expressing, Control

    An external file that holds a picture, illustration, etc. Object name is plantbiotechnology-39-4-22.0926a-figure01.jpg

    Journal: Plant Biotechnology

    Article Title: Recombinant MBP-pσ1 expressed in soybean seeds delays onset and reduces developing disease in an animal model of multiple sclerosis

    doi: 10.5511/plantbiotechnology.22.0926a

    Figure Lengend Snippet: An external file that holds a picture, illustration, etc. Object name is plantbiotechnology-39-4-22.0926a-figure01.jpg

    Article Snippet: Construction of soybean transformation vectors Sequences encoding the Glycine max Gy1 promoter and signal peptide (SP) ( Sims and Goldberg 1989 ), hMBP, and reovirus type 3 sigma1 protein (pσ1) were synthesized by commercial vendors (Geneart and ATUM).

    Techniques: Transformation Assay, Plasmid Preparation, Expressing, Virus, Sequencing, Software, Residue

    An external file that holds a picture, illustration, etc. Object name is plantbiotechnology-39-4-22.0926a-figure02.jpg

    Journal: Plant Biotechnology

    Article Title: Recombinant MBP-pσ1 expressed in soybean seeds delays onset and reduces developing disease in an animal model of multiple sclerosis

    doi: 10.5511/plantbiotechnology.22.0926a

    Figure Lengend Snippet: An external file that holds a picture, illustration, etc. Object name is plantbiotechnology-39-4-22.0926a-figure02.jpg

    Article Snippet: Construction of soybean transformation vectors Sequences encoding the Glycine max Gy1 promoter and signal peptide (SP) ( Sims and Goldberg 1989 ), hMBP, and reovirus type 3 sigma1 protein (pσ1) were synthesized by commercial vendors (Geneart and ATUM).

    Techniques: Enzyme-linked Immunosorbent Assay, Incubation, Transformation Assay, Western Blot, SDS Page, Membrane, Comparison, Transgenic Assay, Clear Native PAGE, In Vitro, Binding Assay, Fluorescence

    An external file that holds a picture, illustration, etc. Object name is plantbiotechnology-39-4-22.0926a-figure03.jpg

    Journal: Plant Biotechnology

    Article Title: Recombinant MBP-pσ1 expressed in soybean seeds delays onset and reduces developing disease in an animal model of multiple sclerosis

    doi: 10.5511/plantbiotechnology.22.0926a

    Figure Lengend Snippet: An external file that holds a picture, illustration, etc. Object name is plantbiotechnology-39-4-22.0926a-figure03.jpg

    Article Snippet: Construction of soybean transformation vectors Sequences encoding the Glycine max Gy1 promoter and signal peptide (SP) ( Sims and Goldberg 1989 ), hMBP, and reovirus type 3 sigma1 protein (pσ1) were synthesized by commercial vendors (Geneart and ATUM).

    Techniques: Western Blot, Clear Native PAGE, Negative Control, Comparison

    An external file that holds a picture, illustration, etc. Object name is plantbiotechnology-39-4-22.0926a-figure04.jpg

    Journal: Plant Biotechnology

    Article Title: Recombinant MBP-pσ1 expressed in soybean seeds delays onset and reduces developing disease in an animal model of multiple sclerosis

    doi: 10.5511/plantbiotechnology.22.0926a

    Figure Lengend Snippet: An external file that holds a picture, illustration, etc. Object name is plantbiotechnology-39-4-22.0926a-figure04.jpg

    Article Snippet: Construction of soybean transformation vectors Sequences encoding the Glycine max Gy1 promoter and signal peptide (SP) ( Sims and Goldberg 1989 ), hMBP, and reovirus type 3 sigma1 protein (pσ1) were synthesized by commercial vendors (Geneart and ATUM).

    Techniques: Formulation, Staining, Bradford Assay, Molecular Weight, Western Blot, Clear Native PAGE